Rat Model Experiment Overview

The first part of animal trials entailed the use of decellularized dermal membranes, derived from adult human donor foreskins through tissue engineering principles, for the reconstruction of tissues and genital organs: in vivo experimental study in an animal model.

Veterinarian Assistants prepare the surgical equipment for the experiment

Veterinarian Assistant checking the vitals of a rat

Recently, Purpura et al. developed an acellular biologic scaffold derived from human donor foreskin.

Unlike other commercial natural and synthetic biomaterial scaffolds, which are less specialized, this decellularized extracellular matrix is characterized by the same intrinsic topography and mechanical properties that are biologically inherent in the foreskin tissue.

  • Rich in FGFb (Basic Fibroblast Growth Factor)
  • Neoangiogenesis
  • Revascularization
  • Tissue Regeneration

The study evaluated the biocompatibility of the membrane–host system through immunological response (tissue heating, redness, and swelling) and degree of tissue repair versus regeneration. The qualitative results of the biomaterial implantation are:

  • Absence of demarcation
  • Abundant cellularization
  • Numerous microvessels
  • No encapsulation

Some select images from the experiment:

Picture of membrane after initial implantation

Picture of membrane at the end of the experiment

Histological slide of integrated membrane

These preliminary findings indicate that our decellularized foreskin dermal membranes, upon subcutaneous implantation, integrated with the host, resulting in minimal immune response and facilitating normal wound healing, which yielded the desired tissue regeneration. These are encouraging results as Foregen moves into the last part of the animal trials phase!